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Product Info Summary
| SKU: | P30408 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Arabidopsis |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Phospho-S6 Kinase 1 (Thr449) ATPK1 Antibody
SKU/Catalog Number
P30408
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-S6 Kinase 1 (Thr449) ATPK1 Antibody (Catalog # P30408). Tested in WB applications. This antibody reacts with Arabidopsis.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-S6 Kinase 1 (Thr449) ATPK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P30408)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Clone Number
1B9
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr449 of Arabidopsis S6K1, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Arabidopsis.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P30408 is reactive to in Arabidopsis
Applications
P30408 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
52 kDa
Calculated molecular weight
52588 MW
Background of
Ribosomal s6 kinase is a member of a family of protein kinases involved in signal transduction. The subfamily S6K has two known homologues: S6K1 and S6K2. First characterized in mammals, S6K1 is controlled by target-of-rapamycin (TOR) kinase, which plays a central regulatory role in growth signaling pathways (Dufner and Thomas 1999). Osmotic stress inhibition of S6K is mediated by the TOR kinase pathway (Mahfouz et al., 2006). The activation of mammalian S6K1 involves phosphorylation at Thr-389 (Pearson et al., 2005), however its orthologue in Arabidopsis suggests that plant S6K1 Thr-449 is its functional equivalent (Schepetilnikov et al., 2011). The phytohormone auxin triggers TOR activation, which is followed by S6K1 phosphorylation at Thr-449, which in turn is critical for translation reinitiation (Schepetilnikov et al., 2013). Rapamycin effectively inactivates S6K1 Thr-449 phosphorylation in Arabidopsis seedlings, which suppresses TOR PK activity and ultimately plant growth (Xiong Y and Sheen J, 2011).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
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Western blot of Arabidopsis lysate showing specific immunolabeling of the ~53 kDa S6K1 phosphorylated at Thr449 in the first lane (-). Phosphospecificity is shown in the second lane (P) where immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide in the third lane (NP).
Specific Publications For Anti-Phospho-S6 Kinase 1 (Thr449) ATPK1 Antibody (P30408)
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No publications found for P30408
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